ETHE1 Knockout cell line (HT-29)

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ETHE1 Knockout cell line (HT-29) Order

ETHE1 Knockout cell line (HT-29)
Catalog#
YKO-H1125
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ETHE1 Knockout cell line (HT-29)
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Detailed Product Information

Product category
KO Cell Lines
cell name
ETHE1 Knockout cell line (HT-29)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
ETHE1
Gene ID
23474
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Accession
CVCL_D9WG(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript ETHE1-201)
Validation: PCR+Sanger Sequencing
ETHE1 Knockout cell line (HT-29) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HT-29		 STR Info (Cell bank)
Cell Line:HT-29
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11121112
D7S820 10101010
D16S539 11121112
VWA 17191719
TH01 6969
AMEL XXXX
TPOX 8989
CSF1PO 11121112
D12S391 18.321
FGA 2022
D2S1338 1923
D21S11 2930
D18S51 1313
D8S1179 1010
D3S1358 1517
D6S1043 1214
PENTAE 1416
D19S433 1414.2
PENTAD 1113
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ETHE1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-29 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ETHE1 gene knockout ETHE1 Knockout cell line (HT-29) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ETHE1 Knockout cell line (HT-29)
ETHE1 Knockout cell line (HT-29)
Catalog#
YKO-H1125
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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